Two-Color Probe to Monitor a Wide Range of pH Values in Cells

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author Jong Seung Kim
journal Angew. Chem. Int. Ed. 2013, 52, 6206-6209


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High-tech pH paper : A “chameleon” pH probe composed of rhodamine (red, see scheme) and fluorescein (green) units emits at wavelengths of 580 nm and 512 nm, where the intensities show a contrary response to pH changes. Confocal microscopy of HeLa cells with this probe reveals red and green spots; the ratio of these signals can be calibrated to give the pH value of the respective organelle.



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