2015.02.25 13:29
Multiphotonics Nanoprobes: Innovative Nanotools for Bioapplications
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| 초청강사 | Dr. Mireille Blanchard-Desce |
|---|---|
| 소속 | Institut des Sciences Moléculaires (CNRS UMR5255), University of Bordeaux, France |
| 일시 | 2015.03.02(월) 오후 4시30분 |
| 장소 | 아산이학관 331 |
Multiphotonics has attracted a lot of interest over recent years thanks to the advantages it provides in microscopic bioimaging. These include an intrinsic three-dimensional resolution, increased penetration depth in tissues and reduced photodamages. To take full benefits of multiphotonics, the design of dedicated probes having very large nonlinear optical responses (such as two-photon absorption (2PA) or second-harmonic generation (SHG) cross-sections) is highly desirable as it opens the way to improved sensitivity, faster scanning speed as well as selective photo-addressing [1a]. As an illustration, examples of multiphotonics probes designed for bioimaging and sensing purposes (pH imaging, subcellular imaging, membrane dynamics, real-time monitoring of neuronal activity, heavy metals detection in effluents….) will be presented [1b-f]. Two-photon activatable phototriggers have also been developed for synchronous photoinitiation of reductive bioprocesses [1a] as well as antenna systems for efficient uncaging of neurotransmitters [2].
Nanoparticles offer major potential as they can afford potentially extremely bright nano-objects with large nonlinear responses. In particular luminescent semiconductor-based nanoparticles (i.e., quantum dots QDs) have been shown to be of interest for 2PEF bioimaging [3]. QDs with large 2PA responses however raise critical toxicity and biodegradability issues inspiring us to develop biocompatible and ecofriendly alternatives. With this aim in mind, we have developed two bottom-up routes towards fully-organic nanoemitters. The first route led to soft tunable organic nanodots (ONDs), having radius of a few nm, which outcome QDs in terms of 1P and 2P brightness [4a], and allow fast excitation energy transfer [4b]. ONDs have proven of major interest for in vivo bio-imaging [4c] as well as for detection of explosives by 2PE [4b]. The second route is based on the spontaneous assembly in water of insoluble dyes. From specifically-designed dyes, we elaborated, via a simple, fast and green protocol, Fluorescent Organic Nanoparticles (FONs), typically 10-50 nm in radius, showing extremely high one- and two-photon brightness (up to 108 M-1cm-1 and 106 GM) [5]. Their color, biocompatibility and colloidal stability can be tuned thanks to a subtle bottom-up strategy, yielding biocompatible ultrasensitive nanotracers for in vivo angiography [5a]. As a result, hyperbright NIR-emitting molecular-based FONs (thus named HiFONs) have been successfully applied to imaging in cells as well as to single particle tracking in water [5b].
References
[1] a) A.-C. Robin, S. Gmouh, O. Mongin, V. Jouikov, M. H. V. Werts, C. Gautier, A Slama-Schwok and M. Blanchard-Desce, Chem. Commun. 2007, 1334; b) L. Ventelon, S. Charier, L. Moreaux, J. Mertz and M. Blanchard-Desce, Angew. Chem., 2001, 40, 2098-2101; b) L. Moreaux, O. Sandre, S. Charpak, M. Blanchard-Desce and J. Mertz, Biophys. J., 2001, 80, 1568-1574; c) D. A. Dombeck, M. Blanchard-Desce and W. W. Webb, J. Neuroscience, 2004, 24, 999-1003; D. A. Dombeck, L Sacconi, M. Blanchard-Desce and W. W. Webb, J. Neurophys., 2005, 94, 3628-3636; L. Moreaux, T. Pons, O. Mongin, M. Blanchard-Desce and J. Mertz, J. Biomed. Optics, 2003, 8, 428-431; d) M. H. V. Werts, S. Gmouh, O. Mongin, T. Pons and M. Blanchard-Desce, J. Amer. Chem. Soc., 2004, 126, 16294-16295; e) C. Le Droumaguet, A. Sourdon, E. Genin, O. Mongin and M. Blanchard-Desce, Chem. Asian J., 2013, 8, 2984-3001; f) J. Bell, I. Samb, P.Y. Toullec, O. Mongin, M. Blanchard-Desce, V. Michelet and I. Leray, New J. Chem., 2014, 38, 1072-1078.
[2] S. Picard, E. Cueto-Diaz, E. Genin, G. Clermont, F. Acher, D. Ogden, M. Blanchard-Desce, Chem. Comm., 2013, 49, 10805-10807; E. J. Cueto Díaz, S. Picard, V. Chevasson, J. Daniel, V. Hugues, O. Mongin, E. Genin, M. Blanchard-Desce, Org. Lett. 2015, 17, 102-105.
[3] D. R. Larson, W. R. Zipfel, R. M. Williams, S. W. Clark, M. P. Bruchez, F. W. Wise and W. W. Webb, Science 2003, 300, 1434-1437.
[4] a) M. Blanchard-Desce, M. Werts, O. Mongin, J.-P. Majoral, A.-M. Caminade, R. K. Thatavarthy, PCT Int. Appl., 2007, WO 2007080176. O. Mongin, C. Rouxel, J.-M. Vabre, Y. Mir, A. Pla-Quintana, Y. Wei, A.-M. Caminade, J. P. Majoral and M. Blanchard-Desce, Proc. SPIE-Int. Soc. Opt. Eng, 2009, 7403, 740303-1-12; b) A. Narayanan, O. Varnavski, O. Mongin, J.-P. Majoral, M. Blanchard-Desce and T. Goodson III, Nanotechnology, 2008, 19, 115502; c) T. R. Krishna, M. Parent, M. H. V. Werts, L. Moreaux, S. Gmouh, S. Charpak, A.-M. Caminade, J.-P. Majoral and M. Blanchard-Desce, Angew. Chem., Int. Ed. 2006, 45, 4645-4648; O. Mongin, C. Rouxel, A.-C. Robin, A. Pla-Quintana, Tathavarathy Rama Krishna, G. Recher, F. Tiaho, A. -M. Caminade, J.-P. Majoral and M. Blanchard-Desce, Proc. SPIE-Int. Soc. Opt. Eng. 2008, 7040, 704006, 1-12.
[5] a) V. Parthasarathy, S. Fery-Forgues, E. Campioli, G. Recher, F. Terenziani and M. Blanchard-Desce, Small, 2011, 7, 3219-3229; b) E. Genin, Z. Gao, J. A. Varela, J. Daniel, T. Bsaibess, I. Gosse, L. Groc, L. Cognet and M. Blanchard-Desce, Adv. Mater. 2014, 26, 2258-2261.
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