Fluorescence imaging for redox-signaling research in cancer cells and role of bioimaging data curation center (BDCC) for FAIR (Findability, Accessibility, Interoperability, and Reuse)

Abstract: 

Exposure to high concentrations of reactive oxygen species results in oxidative stress and oxidative damage, which contributes to numerous pathologies including cancer, inflammatory disease, and ageing. Recent evidence indicates that reactive oxygen species, especially hydrogen peroxide (H₂O₂) have been reported to act as signaling mediators implicated in cell proliferation, differentiation and growth by oxidizing specific thiol proteins. Changes in the levels of a phosphorylated phosphatidylinositide (PtdIns) in cellular organelles often alter the function of the organelles by modifying the PtdIns-dependent recruitment of mediators and effectors. The effect of intracellular hydrogen peroxide (H₂O₂) molecules on phosphoinositide signaling, which is implicated in numerous cellular processes is unclear. Genetically encoded fluorescent reporters - and specifically one engineered using a highly sensitive, selective, and reversible H₂O₂ reporter called Hyper-may be used to monitor the dynamic change of cellular H₂O₂ level. With the use of organelle targeting H₂O₂ reporter and fluorescent phosphorylated PtdIns reporters, we found new roles of H₂O₂ on the regulation of PtdIns signaling in cancer cells. There is a growing need for basic and clinical researchers to access and use curated bioimaging data. I will introduce the roles of the Bioimaging Data Curation Center (BDCC) and the Bioimaging Advanced Platform (BioiAP) which is operated by BDCC. To make these data findable, accessible, interoperable, and reusable (FAIR), K-BDS should be arranged properly. In order to promote collaborative research, the main goal of BDCC is to standardize, organize, and make the vast volumes of data produced by various imaging modalities in the field of life science accessible and reusable. BDCC promotes scientific collaboration by providing a venue for scientists to exchange ideas, interact on projects incorporating bioimaging data, and exchange information. BDCC and BioiAP will provide an essential link between the production of unprocessed bioimaging data and their efficient reuse in scientific research.

20250501_대학원 세미나_강동민 교수.pdf