Chasing Phosphohistidine, “Dark Matter” in the Phosphoproteome
Abstract:
My research program focuses on the synergistic combination of synthetic organic chemistry and protein
chemistry for innovative applications in biology and medicine. I will discuss our recent work on
phosphohistidine (pHis), a labile protein post-translational modification(PTM). Despite the long-recognized
importance of pHis in cell signaling and metabolism,the full scope and exact function of protein histidine
phosphorylation is not clearly understood. It is mainly due to the chronic lack of adequate research tools to
investigate the unstable PTM. We provided first glimpse at this “dark matter” in the phosphoproteome through
the development of novel chemical tools for studying histidine phosphorylation, including stable pHis analogs,
pHis-specific antibodies, and bisubstrate analogs for histidine kinases.
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